Hey class, this is the summary of todays lab and i hope you will understand the lab better after reading this blog entry.
Enzymes catalyze reactios by lowering the activation energy necessary for a reaction to occur.
The molecule that an enzyme acts on is called the substrate. In an enzymatic reaction, the substrate changes and becomes into products but the enzyme molecule is unchanged after the reaction and it can continue to catalyze reactions over and over again.
In part 2: Test of Catalase activity, the enzyme that was used was the catalase, the substrate was H2O2 and the products came out to be water and Oxygen. When the catalase was added to the H2O2 solution oxygen bubbles started to form because catalase is an enzyme which speeded the reaction.
Although in the next step the catalase was increased but it was boiled causing the enzyme to change its shape and it became denatured so nothing happened. There were no oxygen bubbles observed. Also, in the last step of this part of the experiment, when the chicken liver was added to the H2O2 huge amount of Oxygen was released because alot of oxygen bubbles appeared. Here is a similar video of chicken liver and H2O2 being mixed.
So, from this part of the experiment it could be concluded that the living tissue produced the most oxygen and it had the fastest reaction rate.
For part 3, the purpose of the lab was to investigate the rate at which the enzyme catalase converts substrate to product. The catalase reacted with H2O2 for various amount of tie and then it was stopped by adding the H2SO4. So the remaining H2O2 needed to be measured so a titration with KMNO4 took place. Here is an overall image of the experiment.
In the overall results it could be concluded that as time passed the number of moles of product also increased.
well, thats it, i hope u guys learned something and hopefully it wasn't that boring. Ok now for the moment everyone has been waiting for, the next sherpa who will have the honor of leading us is............... Aerialle.
